Methods for identifying LILRB-blocking antibodies
AssigneeBoard of Regents, The University of Texas System
InventorsChengcheng Zhang, Zhiqiang An, Ningyan Zhang, Mi Deng, Jaehyup Kim, Xun Gui
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Abstract
Provided herein are methods and compositions for the identification of modulators of ApoE-induced LILRB activation. Also provided herein are methods of treating cancer comprising the administration of an inhibitor of ApoE-induced LILRB activation. Also provided are methods of treating autoimmune disease or inhibiting the onset of transplant rejection or treating an inflammatory disorder comprising administering an agonist of ApoE-induced LILRB activation to a subject.
Claims26
A method of identifying a modulator of Leukocyte Immunoglobulin-Like Receptor B4 (LILRB4) activation comprising:
- (a) contacting a reporter cell with Apolipoprotein E (ApoE) and a candidate substance, wherein the reporter cell expresses a reporter gene that encodes a detectable reporter operably linked to a promoter regulated by activation of LILRB4;
- and
- (b) detecting a level of LILRB4 activation in the reporter cell, wherein an increase or decrease in the level of LILRB4 activation as compared to a reference level measured from a reporter cell not treated with said candidate substance indicates that the candidate substance is a modulator of LILRB4 activation in the reporter cell.
Dependencies are inferred from the claim text.