Metabolic profiling by reverse-phase/ion-exchange mass spectrometry
AssigneeStanford Junior University
InventorAnthony T. Le
Life of this patent
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Abstract
Disclosed herein are methods of analyzing a biological sample comprising: separating components of the biological sample via reversed-phase (RP) chromatography to obtain an elute; subjecting the elute to separation via ion-exchange (IEX) chromatography or mixed-mode IEX chromatography; and detecting the separated compounds to determine the components of the biological sample. Also disclosed are devices comprising a reversed-phase (RP) chromatography column in communication with an ion-exchange (IEX) chromatography column or mixed-mode IEX chromatography column, wherein there is no switching valve between the columns.
Claims11
A method of analyzing a biological sample comprising:
- separating components of the biological sample via reversed-phase (RP) chromatography to obtain an elute;
- subsequently, subjecting the elute to separation via ion-exchange (IEX) chromatography or mixed-mode IEX chromatography;
- and
- detecting separated compounds to determine the components of the biological sample;
- wherein:
- the separating and the subjecting are conducted with one aqueous to organic solvent gradient;
- and
- there is no switching valve between the RP chromatography and IEX chromatography.
Dependencies are inferred from the claim text.