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US 11,906,491 B2Granted
Peptide markers for authenticating Ejiao and related gelatins
AssigneeHong Kong Baptist University
InventorsQuanbin Han, Wenjie Wu, Lifeng Li
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Abstract
Provided herein is a method for authenticating animal hide gelatins using peptide markers found animal hide gelatin hydrolysates. The method can be used to authenticate animal hide gelatins derived from donkey hide, horse hide, pig hide, and cattle hide.
Claims13
A method of identifying an animal hide gelatin in a sample, the method comprising:
- providing a hydrolysate of the sample;
- analyzing the hydrolysate of the sample using a mass spectroscopy method;
- and
- detecting one or more peptide markers, wherein a donkey hide gelatin is identified when the one or more peptide markers have an observed mass to charge ratio (m/z) selected from the group consisting of:
- 518.2695±0.1 (DM1), 675.6632±0.1 (DM2), 761.3672±0.1 (DM3), 766.6952±0.1 (DM4), 774.0218±0.1 (DM5), 784.3392±0.1 (DM6), and 854.0603±0.1 (DM7);
- or a pig hide gelatin is present when two or more peptide markers have an observed mass to charge ratio (m/z) selected from the group consisting of:
- 419.2446±0.1 (PM1), 490.5942±0.1 (PM2), 693.8432±0.1 (PM3), 713.6902±0.1 (PM4), 773.9237±0.1 (PM5), and 774.9121±0.2 (PM6).
4 more elements
Dependencies are inferred from the claim text.