Spectrofluorophotometer, spectrofluoro-measurement method, and image capturing method

A spectrofluoro-measurement method using a spectrofluorophotometer comprising

• a light source,
• an excitation-side spectroscope that performs spectral dispersion from light of the light source and generates excitation light,
• a fluorescence-side spectroscope that performs spectral dispersion of fluorescence emitted from a sample irradiated with the excitation light into monochromatic light,
• a sample container installation portion for holding a sample container which receives a liquid sample and is formed of a transparent material,
• a detector that detects fluorescence emitted from the liquid sample, and
• an image capturing device that captures a sample image of the liquid sample emitting fluorescence,
• the spectrofluoro-measurement method comprising:
• causing excitation light to be directly incident in an excitation light incidence direction through a port provided in the excitation light incidence direction of the sample container installation portion;
• causing excitation light that has been transmitted through the sample in a linear direction to be transmitted through a port provided on a surface facing the port in the excitation light incidence direction to outside;
• causing the detector to detect fluorescence through a port provided in a fluorescence emission direction of the sample container installation portion;
• and
• causing the image capturing device to capture the sample image via a port provided in another direction of the sample container installation portion.

An image capturing method using a spectrofluorophotometer comprising:

• irradiating a sample with excitation light;
• blocking excitation light after fluorescence and phosphorescence are emitted from the sample;
• detecting only phosphorescence emitted from the sample after excitation light is blocked;
• and
• capturing a sample image in synchronization with a detection timing of phosphorescence.