Method and product for preparing a protein-containing sample for analysis by mass spectrometry
AssigneeThermo Finnigan LLC
InventorsDaniel Lopez Ferrer, Aaron M. Robitaille, Michael O. Krawitzky, Greg A. Foster
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Abstract
A method for preparing a protein-containing sample for analysis by mass spectrometry includes introducing the sample into a reaction vessel. The reaction vessel contains a reagent mixture including pre-measured quantities of an immobilized proteolytic enzyme, a reducing agent and an alkylating agent. The contents of the reaction vessel are activated by heating or by sonication.
Claims19
A product for preparing a protein-containing sample for mass spectrometry analysis, comprising:
- a closeable reaction vessel having an internal volume;
- and
- a reagent mixture disposed within the internal volume of the closeable reaction vessel and comprising:
- an immobilized proteolytic enzyme;
- a reducing agent;
- and
- an alkylating agent, wherein the reducing agent is at a concentration between 1 mM and 5 mM, and the alkylating agent is at a concentration between 10 mM and 25 mM.
A method for manufacturing a product for preparing a protein-containing sample for mass spectrometry analysis, comprising:
- providing a closeable reaction vessel having an internal volume;
- introducing a reagent mixture into the internal volume of the closeable reaction vessel, wherein the reagent mixture comprises:
- an immobilized proteolytic enzyme;
- a reducing agent;
- and
- an alkylating agent, wherein the reducing agent is tris(2-carboxyethyl) phosphine (TCEP) at a concentration between 1 mM and 5 mM, and wherein the alkylating agent is one of:
- chloroacetamide (CAA) at a concentration between 10 mM and 25 mM;
- iodacetic acid (IAC) at a concentration between 1 mM and 5 mM;
- and Iodoacetamide (IAA) at a concentration between 1 mM and 15 mM.
A product for preparing a protein-containing sample for mass spectrometry analysis, comprising:
- a closeable reaction vessel having an internal volume;
- and
- a reagent mixture disposed within the internal volume of the closeable reaction vessel and comprising:
- an immobilized proteolytic enzyme;
- a reducing agent;
- and
- an alkylating agent, wherein the reducing agent is tris(2-carboxyethyl) phosphine (TCEP) at a concentration between 1 mM and 5 mM, and wherein the alkylating agent is one of:
- chloroacetamide (CAA) at a concentration between 10 mM and 25 mM;
- iodacetic acid (IAC) at a concentration between 1 mM and 5 mM;
- and Iodoacetamide (IAA) at a concentration between 1 mM and 15 mM.
Dependencies are inferred from the claim text.