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US 11,898,194 B2Granted
Method for detecting target molecule
AssigneeToppan Printing Co., Ltd.
InventorsTakumi Hirase, Yoichi Makino, Yosuke Horiuchi
Life of this patent
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Abstract
A method for detecting a target molecule, including supplying a liquid dispersion of a structural substance including a target molecule into a well array such that the structural substance is introduced into wells of the well array, supplying a sealing solution into the well array such that a layer of the sealing solution is formed on the liquid dispersion in the wells, and that the liquid dispersion is sealed in the wells, extracting the target molecule from the structural substance, and detecting the target molecule.
Claims20
A method for detecting a target molecule, comprising:
- providing a fluid device having a flow path and a plurality of wells arrayed in the fluid device and connected to the flow path;
- supplying a liquid dispersion of a structural substance having a target molecule into the fluid device such that the structural substance is distributed into the plurality of wells in the fluid device and has the target molecule in or on the structural substance;
- supplying a sealing solution into the fluid device such that a layer of the sealing solution is formed in the flow path in the fluid device and individually seals the plurality of wells containing the liquid dispersion;
- extracting the target molecule from the structural substance in the plurality of wells sealed by the layer of the sealing solution in the fluid device such that the liquid dispersion in the plurality of wells includes the structural substance and the target molecule extracted from the structural substance;
- and
- detecting the target molecule extracted from the structural substance in the plurality of wells in the fluid device,
- wherein the fluid device includes a substrate and a cover member attached to the substrate such that the plurality of wells is formed in the substrate and that, the flow path is formed between the substrate and the cover member, and the supplying of the sealing solution includes forming the layer of the sealing solution between the substrate and the cover member such that the layer of the sealing solution entirely fills the flow path formed between the substrate and the cover member.
4 more elements
Dependencies are inferred from the claim text.