Back to results
US 11,898,193 B2Granted
Manipulating droplet size
AssigneeBio-Rad Laboratories, Inc.
InventorsBenjamin J. Miller, Qun Zhong, Darren Roy Link
Life of this patent
Estimating the term…
Abstract
The invention generally relates to methods and systems for manipulating droplet size. In certain aspects, the invention provides methods for manipulating droplet size that include forming droplets of aqueous fluid surrounded by an immiscible carrier fluid, and manipulating droplet size during the forming step by adjusting pressure exerted on the aqueous fluid or the carrier fluid.
Claims14
A system for forming droplets, the system comprising:
- a microfluidic substrate comprising a sample channel and a carrier fluid channel that connects to the sample channel at a junction;
- a manifold in fluid communication with the microfluidic substrate, the manifold configured to deliver an aqueous fluid to the sample channel and an immiscible carrier fluid to the carrier channel to thereby form droplets of aqueous fluid surrounded by carrier fluid at the junction;
- an imager configured to capture images of the droplets;
- and
- a pump in fluid communication with the microfluidic substrate, the pump configured to regulate, based on the images of the droplets, pressure of the aqueous fluid at the junction to produce droplets of substantially uniform size.
2 more elements
Dependencies are inferred from the claim text.