Synergistic genome-nonintegrating reprogramming by microRNAs and transcription factors

A method of generating mammalian patient-specific induced pluripotent stem cells for treating a patient, comprising:

• providing a quantity of mammalian somatic or non-embryonic cells from the patient;
• contacting the mammalian somatic or non-embryonic cells with a quantity of one or more reprogramming factors and one or more microRNA molecules;
• and
• culturing the somatic or non-embryonic cells for a period of time sufficient to generate at least one induced pluripotent stem cell,
• wherein the one or more microRNA molecules consist of (i) at least one miR-302 cluster member and (ii) at least one miR-200 cluster member, and
• wherein the quantity of reprogramming factors comprises at least Oct-4, Sox-2, Klf-4, and at least one of c-Myc or 1-Myc.