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US 11,898,167 B2Granted
Reversible stencils for fabricating micro-tissues
AssigneesThe J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Gladstone, The Regents of the University of California
InventorsNathaniel Huebsch, Bruce Conklin, Kevin E. Healy, Peter Loskill
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Abstract
The invention relates to devices, methods, kits, and compositions for in vitro generation of three-dimensional micro-tissues that are accurate models of heart, skeletal muscle, neuronal, and other tissues.
Claims22
A device for confining mammalian cells, forcing cellular/tissue alignment and tissue self-assembly, comprising:
- a cell adhesion substrate;
- and
- a removable elastomeric stencil overlay;
- wherein the elastomeric stencil has one or more cut-out patterned microwells comprising two or more circular, oval, rectangular, square, V-shaped, or triangular holes, each hole joined to an adjacent hole by a canal (shaft);
- and
- wherein the cell adhesion substrate binds cells within the holes and the canals of the cut-out pattern;
- and
- wherein at least one of the microwells has at least two square holes with a side length “L” of 250 μm to 1000 μm, and the canal joining the two square holes has a longitudinal length “X” of 250-1000 μm and a traverse width “Y” of 50-200 μm.
5 more elements
Dependencies are inferred from the claim text.