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US 11,898,165 B2Granted
Engineered host cells and methods of use thereof
AssigneeMiNK Therapeutics, Inc.
InventorsMarc Van Dijk, Volker Seibert, Robert Benjamin Stein
Life of this patent
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Abstract
Provided herein are engineered host cells suitable for expression of functional T cell receptors (TCR) and methods of using these cells to identify TCRs that specifically bind to a desired peptide-major histocompatibility complex (MHC) complex.
Claims12
An isolated cell derived from a mouse lymphocyte, the isolated cell expressing a chimeric CD8 on the cell surface, wherein the isolated cell comprises:
- (a) a first polynucleotide encoding a chimeric CD8α chain comprising an extracellular region of human CD8α and transmembrane and cytoplasmic regions of mouse CD8α,
- (b) a second polynucleotide encoding a chimeric CD8β chain comprising an extracellular region of human CD8β and transmembrane and cytoplasmic regions of mouse CD8β, wherein the isolated cell expresses CD3 but does not express endogenous TCR on the cell surface;
- and
- (c) one or more reporter system for measurement of TCR signaling;
- wherein the chimeric CD8α chain comprises the amino acid sequence of SEQ ID NO:
- 1, and/or the chimeric CD8β chain comprises the amino acid sequence of SEQ ID NO:
- 2.
4 more elements
Dependencies are inferred from the claim text.