Synthetic near-threshold translational repressors
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsAlexander Green, Yu Zhou
Life of this patent
Estimating the term…
Abstract
Provided herein are synthetic nucleic acid molecules and methods of using such synthetic nucleic acid molecules for strong repression of target gene expression. In particular, provided herein are methods for altering expression of a protein in a cell, where the method comprises introducing into a cell a protein coding sequence operably linked to a near-threshold translational repressor having first and second trigger recognition sequences that are fully or partially complementary to a repressing trigger RNA; and introducing into a cell the repressing trigger RNA.
Claims6
A synthetic nucleic acid molecule comprising a NOT-OR (NOR) logic circuit, the NOR logic circuit comprising:
- at least one input RNA sensing hairpin module comprising:
- an input RNA binding domain;
- and
- a loop domain;
- wherein the input RNA binding domain is complementary to an input RNA;
- and wherein the loop domain comprises first and second trigger RNA sequences;
- a near-threshold translational repressor (NeaTTR) hairpin module comprising:
- a loop-forming region comprising a ribosomal binding site (RBS);
- and
- a stem-forming region comprising a start codon;
- and
- first and second trigger recognition sequences located 5′ and 3′ to the NeaTTR hairpin module, respectively;
- wherein the first and second trigger recognition sequences are complementary to the first and second trigger RNA sequences, respectively, of the input RNA sending hairpin module.
Dependencies are inferred from the claim text.