Tissue processing apparatus and method for processing adipose tissue

A method for preparing a product for treatment of osteoarthritis in a human patient with uncultured stromal vascular fraction cells derived from harvested adipose tissue from the patient, the method comprising:

• washing a volume of harvested adipose tissue from the patient within a single-use, portable processing container to remove blood components from the harvested adipose tissue and prepare washed adipose tissue;
• after the washing, enzymatically digesting the washed adipose tissue within the processing container, the enzymatically digesting comprising combining the washed adipose tissue and an enzyme-containing digestion medium in a mixture and agitating the mixture for a digestion retention time to release stromal vascular fraction cells from the washed adipose tissue;
• after the enzymatically digesting, centrifuging the processing container to form in the processing container a density-separated pellet phase of stromal vascular fraction cell concentrate comprising stromal vascular fraction cells released from the washed adipose tissue during the enzymatically digesting;
• after the centrifuging, selectively removing at least a portion of the stromal vascular fraction cell concentrate from the processing container;
• and
• disposing in a syringe an osteoarthritis treatment formulation comprising a quantity of the stromal vascular fraction cell concentrate removed from the processing container dispersed in a liquid dispersion medium, wherein the syringe is configured to administer by injection the osteoarthritis treatment formulation to the patient in one or more injections for treatment of the osteoarthritis at one or more joints of the patient;
• and
• the osteoarthritis treatment formulation in the syringe comprises a volume of from 0.25 milliliter to 1.5 milliliters of the stromal vascular fraction cell concentrate.