Anti-TSPAN8/anti-CD3 bispecific antibody and anti-TSPAN8 antibody

A bispecific antibody that binds to TSPAN8 and CD3, comprising:

• (a) a Fab region of an anti-TSPAN8 antibody consisting of:
• a heavy chain fragment comprising a heavy chain variable region of the anti-TSPAN8 antibody;
• and a light chain comprising a light chain variable region of the anti-TSPAN8 antibody;
• (b) an anti-CD3-scFv region comprising a heavy chain variable region and a light chain variable region of an anti-CD3 antibody;
• and
• (c) a Fc region consisting of a first Fc polypeptide linked to the heavy chain fragment of the Fab region (a) and a second Fc polypeptide linked to the anti-CD3-scFv region (b),
• wherein
• the heavy chain variable region of the anti-TSPAN8 antibody comprises a CDR1 consisting amino acids 31 to 35 of SEQ ID NO:
• 6, a CDR2 consisting of amino acids 50 to 66 of SEQ ID NO:
• 6, and a CDR3 consisting of amino acids 99 to 110 of SEQ ID NO:
• 6, and the light chain variable region of the anti-TSPAN8 antibody comprises a CDR1 consisting of amino acids 24 to 34 of SEQ ID NO:
• 8, a CDR2 consisting of amino acids 50 to 56 of SEQ ID NO:
• 8, and a CDR3 consisting of amino acids 89 to 96 of SEQ ID NO:
• 8;
• or
• the heavy chain variable region of the anti-TSPAN8 antibody comprises a CDR1 consisting of amino acids 31 to 35 of SEQ ID NO:
• 10, a CDR2 consisting of amino acids 50 to 66 of SEQ ID NO:
• 10, and a CDR3 consisting of amino acids 99 to 110 of SEQ ID NO:
• 10, and the light chain variable region of the anti-TSPAN8 antibody comprises a CDR1 consisting of amino acids 24 to 34 of SEQ ID NO:
• 12, a CDR2 consisting of amino acids 50 to 56 of SEQ ID NO:
• 12, and a CDR3 consisting of amino acids 89 to 96 of SEQ ID NO:
• 12.