Polypeptides comprising immunoglobulin single variable domains targeting IL-6 and TNF-α

A polypeptide, or a composition comprising the polypeptide, wherein the polypeptide comprises at least three immunoglobulin single variable domains (ISVDs), wherein each of said ISVDs comprises three complementarity determining regions (CDR1 to CDR3, respectively), optionally linked via one or more peptidic linkers;

• and wherein:
• a) a first ISVD binds to IL-6 and comprises
• i. a CDR1 which has the amino acid sequence of SEQ ID NO:
• 6;
• ii. a CDR2 which has the amino acid sequence of SEQ ID NO:
• 10 amino acid difference(s) with SEQ ID NO:
• 10;
• and
• iii. a CDR3 which has the amino acid sequence of SEQ ID NO:
• 14;
• b) a second ISVD binds to IL-6 and comprises;
• iv. a CDR1 which has the amino acid sequence of SEQ ID NO:
• 8
• v. a CDR2 which has the amino acid sequence of SEQ ID NO:
• 12 amino acid difference(s) with SEQ ID NO:
• 12;
• and
• vi. a CDR3 which has the amino acid sequence of SEQ ID NO:
• 16;
• and
• c) a third ISVD binds to TNF-α and comprises
• vii. a CDR1 which has the amino acid sequence of SEQ ID NO:
• 9;
• viii. a CDR2 which has the amino acid sequence of SEQ ID NO:
• 13 amino acid difference(s) with SEQ ID NO:
• 13;
• and
• ix. a CDR3 which has the amino acid sequence of SEQ ID NO:
• 17.