Methods of inhibiting complement factor H (CFH) comprising administering an antibody that binds CFH
AssigneeDuke University
InventorsEdward F. Patz, Jr., Michael J. Campa, Elizabeth Gottlin, Barton F. Haynes, Hua-Xin Liao, M. Anthony Moody
Life of this patent
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Abstract
Disclosed herein are Complement factor H (CFH) inhibitors, such as anti-CFH antibodies and small molecules, and methods of using said inhibitors.
Claims17
A method for inhibiting complement factor H (CFH) protein in a subject by administering a therapeutically effective amount of an isolated antibody or antibody fragment thereof which immunospecifically binds to Complement Factor H (CFH) protein, wherein the isolated antibody or antibody fragment thereof binds to an epitope of PIDNGDIT (SEQ ID NO:
- 3) within short consensus repeat (SCR) 19 of CFH protein, wherein the antibody comprises:
- (a) a variable heavy domain comprising the amino acid sequence of SEQ ID NO:
- 73 and a variable light domain region comprising the amino acid sequence of SEQ ID NO:
- 84;
- (b) a variable heavy domain comprising the amino acid sequence of SEQ ID NO:
- 74 and a variable light domain region comprising the amino acid sequence of SEQ ID NO:
- 83;
- (c) a variable heavy domain comprising the amino acid sequence of SEQ ID NO:
- 75 and a variable light domain region comprising the amino acid sequence of SEQ ID NO:
- 85;
- (d) a variable heavy domain comprising the amino acid sequence of SEQ ID NO:
- 78 and a variable light domain region comprising the amino acid sequence of SEQ ID NO:
- 88;
- (e) a variable heavy domain comprising the amino acid sequence of SEQ ID NO:
- 79 and a variable light domain region comprising the amino acid sequence of SEQ ID NO:
- 89;
- (f) a variable heavy domain comprising the amino acid sequence of SEQ ID NO:
- 76 and a variable light domain region comprising the amino acid sequence of SEQ ID NO:
- 86;
- or
- (g) a variable heavy domain comprising the amino acid sequence of SEQ ID NO:
- 77 and a variable light domain region comprising the amino acid sequence of SEQ ID NO:
- 87.
A method for increasing complement dependent lysis of a cell, the method comprising administering to a cell a therapeutically effective amount of an isolated antibody or antibody fragment thereof which immunospecifically binds to Complement Factor H (CFH) protein, wherein the isolated antibody or antibody fragment thereof binds to an epitope of PIDNGDIT (SEQ ID NO:
- 3) within short consensus repeat (SCR) 19 of CFH protein, wherein the antibody comprises:
- (a) a variable heavy domain comprising the amino acid sequence of SEQ ID NO:
- 73 and a variable light domain region comprising the amino acid sequence of SEQ ID NO:
- 84;
- (b) a variable heavy domain comprising the amino acid sequence of SEQ ID NO:
- 74 and a variable light domain region comprising the amino acid sequence of SEQ ID NO:
- 83;
- (c) a variable heavy domain comprising the amino acid sequence of SEQ ID NO:
- 75 and a variable light domain region comprising the amino acid sequence of SEQ ID NO:
- 85;
- (d) a variable heavy domain comprising the amino acid sequence of SEQ ID NO:
- 78 and a variable light domain region comprising the amino acid sequence of SEQ ID NO:
- 88;
- (e) a variable heavy domain comprising the amino acid sequence of SEQ ID NO:
- 79 and a variable light domain region comprising the amino acid sequence of SEQ ID NO:
- 89;
- (f) a variable heavy domain comprising the amino acid sequence of SEQ ID NO:
- 76 and a variable light domain region comprising the amino acid sequence of SEQ ID NO:
- 86;
- or
- (g) a variable heavy domain comprising the amino acid sequence of SEQ ID NO:
- 77 and a variable light domain region comprising the amino acid sequence of SEQ ID NO:
- 87.
Dependencies are inferred from the claim text.