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US 11,897,917 B2Granted
Bioconjugation of polypeptides
AssigneeThe University of York
InventorsMartin A. Fascione, Richard J. Spears, Robin Brabham, Darshita Budhadev, Tessa Keenan
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Abstract
Certain embodiments of the present invention relate to methods of forming and manipulating bioconjugates. Particularly, but not exclusively certain embodiments relate to methods of reversible carbon-carbon bond bioconjugation using aldol based chemical reactions at physiological conditions.
Claims21
A method of modifying a polypeptide, the method comprising:
- a. contacting a first polypeptide comprising a first aldehyde moiety with:
- i. an aldehyde donor molecule comprising a second aldehyde moiety;
- and
- ii. a catalyst molecule, under conditions sufficient for an aldol reaction to occur between the first aldehyde group and the second aldehyde group, such that a second polypeptide is formed;
- wherein the second polypeptide comprises a third aldehyde moiety which is a beta-hydroxy aldehyde moiety and further wherein the catalyst molecule comprises a secondary amine moiety or an acid addition salt thereof.
2 more elements
Dependencies are inferred from the claim text.