Aptamer based portable diagnostic medical device and methods of use

A device for detecting and measuring one or more biomarker in a biological sample from a subject, the device comprising:

• a sample access component having at least one microneedle that is adapted to access the biological sample;
• a sample pre-processing component having at least one first aptamer-based magnetic bead conjugate (“aptamer-MB”), the aptamer-MB having a pre-processing aptamer that is specifically configured to chemically bind to non-desired biomarkers, the non-desired biomarkers being different from the one or more biomarker;
• wherein the sample pre-processing component further comprises a pre-processing filter wherein the filter has a pore size that is no larger than the largest biomarker being assessed;
• a sensor component in fluid communication with the sample access component, the sensor component having at least one aptamer that is configured to chemically bind to the one or more biomarker as the biological sample passes through the sensor component, the at least one aptamer being further configured (i) to generate a first resonance frequency prior to being chemically bound to the one or more biomarker and (ii) to generate a second resonance frequency, different from the first resonance frequency, subsequent to being chemically bound to the one or more biomarker;
• and
• an electronic component that is configured to collect, analyze, and compute data received from the sensor component, such as the first and second resonance frequencies, in order to determine the presence of the one or more biomarker within the biological sample.