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US 11,896,005 B2Granted
Warming cryopreserved bone
AssigneeOssium Health, Inc.
InventorsErik J. Woods, Brian H. Johnstone
Life of this patent
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Abstract
The present disclosure relates to, at least, a vacuum-assisted method for infiltrating cadaver bone with a cryoprotectant and a method for rapidly warming the cryopreserved cadaver bone for bone marrow processing.
Claims22
A method for warming cadaver bone for providing bone marrow or a derivative thereof, the method comprising:
- obtaining a cryopreserved cadaver bone;
- dividing the cryopreserved cadaver bone to obtain fragments of the cryopreserved bone;
- and
- transferring the fragments of the cryopreserved bone into a grinding medium having a temperature of from about 35° C. to 45° C. for a time sufficient to warm the cadaver bone fragments to a surface temperature of about 20° C.
1 more element
A method comprising:
- (a) placing a cadaver bone in a closed container comprising a cryoprotectant solution;
- (b) reducing the pressure in the closed container and, optionally, holding the closed container at reduced pressure, to remove at least a portion of the water present in the cadaver bone;
- (c) raising the pressure in the closed container and holding the closed container at a raised pressure to allow infiltration of the cryoprotectant solution into the cadaver bone;
- (d) removing the cadaver bone from the closed container;
- (e) chilling the cadaver bone to a temperature of at least below 0° C., thereby cryopreserving the cadaver bone;
- (f) storing the cryopreserved cadaver bone at a temperature of at least below 0° C.;
- (g) dividing the cryopreserved cadaver bone to obtain fragments of the cryopreserved bone;
- and
- (h) transferring the fragments of the cryopreserved bone into a grinding medium having a temperature of from about 35° C. to about 45° C. for a time sufficient to warm the cadaver bone fragments to a surface temperature of about 20° C.
6 more elements
Dependencies are inferred from the claim text.