CGMMV resistant Citrullus plants
AssigneeNunhems B.V.
InventorsAlberto Sirizzotti, Daniel Bellon Dona, Richard Bernard Berentsen, Daniel Jimenez Fernandez, Courtney Hu, Daniel Puglisi
Life of this patent
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Abstract
The present invention relates to cultivated watermelon plants comprising CGMMV resistance due to introgression of QTLs from a CGMMV resistant donor of the species Citrullus colocynthis.
Claims15
A cultivated watermelon plant or plant cell comprising an introgression fragment on chromosome 9 and/or on chromosome 7 of a CGMMV resistant donor plant of the species Citrullus colocynthis, wherein the introgression fragment on chromosome 9 comprises a CGMMV resistance QTL from a C. colocynthis donor of which a representative sample has been deposited under accession number NCIMB 42624 and wherein the QTL is in-between marker SNP FM9.1 at nucleotide 71 of SEQ ID NO:
- 34 or 35, corresponding to nucleotide 35,675,266 of chromosome 9 of the Watermelon Charleston Grey genome and marker InDel9.1 at nucleotide 91 of SEQ ID NO:
- 36 or at nucleotide 91 of SEQ ID NO:
- 37, corresponding to nucleotide 36,439,966 of chromosome 9 of the Watermelon Charleston Grey genome, and wherein the introgression fragment on chromosome 7 comprises a CGMMV resistance QTL from a C. colocynthis donor of which a representative sample has been deposited under accession number NCIMB 42624 and wherein the QTL is in-between marker InDel7.1 at nucleotide 91 of SEQ ID NO:
- 30 or at nucleotide 91 of SEQ ID NO:
- 31, corresponding to nucleotide 7,022,773 of chromosome 7 of the Watermelon Charleston Grey genome and marker SNP FM7.1 at nucleotide 71 of SEQ ID NO:
- 32 or 33, corresponding to nucleotide 8,733,604 of chromosome 7 of the Watermelon Charleston Grey genome.
A method of screening plants or plant parts or seeds, or DNA derived therefrom, for the presence of a fragment on chromosome 7 comprising a CGMMV resistance QTL and/or on chromosome 9 comprising a CGMMV resistance QTL conferring CGMMV resistance comprises the steps of:
- a) screening the genomic DNA for the SNP genotype of one or more or all of SNP13 at nucleotide 51 of SEQ ID NO:
- 13, InDel7.1 at nucleotide 91 of SEQ ID NO:
- 30 or at nucleotide 91 of SEQ ID NO:
- 31, corresponding to nucleotide 7,022,773 of chromosome 7 of the Watermelon Charleston Grey genome, SNP14 at nucleotide 51 of SEQ ID NO:
- 14, SNP15 at nucleotide 51 of SEQ ID NO:
- 15, SNP FM7.1 at nucleotide 71 of SEQ ID NO:
- 32 or 33, corresponding to nucleotide 8,733,604 of chromosome 7 of the Watermelon Charleston Grey genome, SNP16 at nucleotide 51 of SEQ ID NO:
- 16, SNP17 at nucleotide 51 of SEQ ID NO:
- 17, SNP18 at nucleotide 51 of SEQ ID NO:
- 18, linked to the QTL on chromosome 7, and/or for one or more of SNP19 at nucleotide 51 of SEQ ID NO:
- 19, SNP20 at nucleotide 51 of SEQ ID NO:
- 20, SNP21 at nucleotide 51 of SEQ ID NO:
- 21, SNP22 at nucleotide 51 of SEQ ID NO:
- 22, SNP FM9.1 at nucleotide 71 of SEQ ID NO:
- 34 or 35, corresponding to nucleotide 35,675,266 of chromosome 9 of the Watermelon Charleston Grey genome, InDel9.1 at nucleotide 91 of SEQ ID NO:
- 36 or at nucleotide 91 of SEQ ID NO:
- 37, corresponding to nucleotide 36,439,966 of chromosome 9 of the Watermelon Charleston Grey genome, SNP23 at nucleotide 51 of SEQ ID NO:
- 23 and SNP24 at nucleotide 51 of SEQ ID NO:
- 25, linked to the QTL on chromosome 9;
- and
- b) optionally selecting a plant or plant part which comprises on chromosome 9 a Cytosine for marker SNP FM9.1 at nucleotide 71 of SEQ ID NO:
- 34 or 35, corresponding to nucleotide 35,675,266 of chromosome 9 of the Watermelon Charleston Grey genome and/or a Guanine for marker InDel9.1 instead of a Guanine-Cytosine at nucleotide 91 of SEQ ID NO:
- 36 or at nucleotide 91 of SEQ ID NO:
- 37, corresponding to a deletion of the Cytosine at nucleotide 36,439,966 of chromosome 9 of the Watermelon Charleston Grey genome, or selecting a plant or plant part which comprises on chromosome 7 an Adenine for marker InDel7.1 instead of an Adenine-Thymine at nucleotide 91 of SEQ ID NO:
- 30 or at nucleotide 91 of SEQ ID NO:
- 31, corresponding to nucleotide 7,022,773 of chromosome 7 of the Watermelon Charleston Grey genome and/or an Adenine at nucleotide 51 of SEQ ID NO:
- 14 or an Adenine at the equivalent position in a sequence comprising at least 95% sequence identity to SEQ ID NO:
- 14, and/or a Guanine at nucleotide 51 of SEQ ID NO:
- 15 or a Guanine at the equivalent position in a sequence comprising at least 95% sequence identity to SEQ ID NO:
- 15, and/or an Adenine for marker SNP FM7.1 at nucleotide 71 of SEQ ID NO:
- 32 or 33, corresponding to nucleotide 8,733,604 of chromosome 7 of the Watermelon Charleston Grey genome.
Dependencies are inferred from the claim text.