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US 11,889,847 B2Granted
Method for producing a coffee extract employing enzymes having beta-1,3-galactanase activity
AssigneeNovozymes A/S
InventorsNikolaj Spodsberg, Kristian Bertel Roemer M Krogh, Rune Nygaard Monrad, Jens Ekloef, Louise Rasmussen, Gitte Budolfsen Lynglev, Laure Coulomb
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Abstract
The present invention relates to a method for producing a coffee extract which comprises use of an enzyme having β-1,3-galactanase activity and to a coffee extract which comprises at least 20% based on the total weight of soluble coffee solids of total galactose.
Claims10
A method for producing a coffee extract which comprises at least 20% total galactose based on the total weight of soluble coffee solids, comprising the steps:
- (a) adding water, an endo-β-1,3-galactanase (EC 3.2.1.181), and a β-mannanase to roasted and ground coffee beans, wherein the endo-β-1,3-galactanase is added at a concentration of at least 0.001 g/kg coffee beans and a β-mannanase is added in an amount to produce an increased yield of solubilised coffee solids in the coffee extract which is greater than additive as compared to the increased yield of solubilised coffee solids obtained by incubation with each enzyme alone;
- (b) incubating to make an aqueous coffee extract;
- and
- (c) separating the coffee extract from the coffee beans;
- wherein the coffee extract comprises at least 20% total galactose, based on the total weight of soluble coffee solids.
2 more elements
Dependencies are inferred from the claim text.