Continuous stable cell line for identification of infectious African swine fever virus in clinical samples
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsDouglas P. Gladue, Ayushi Rai, Manuel V. Borca
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Abstract
Methods are provided herein utilizing a stable cell line capable of efficient infection by African swine fever virus (ASFV) and also provides for the detection of the presence of virus in samples applied to the cells. Detection of the virus by means such as red blood cell rosetting is a surprising result given that the cell line is derived from African green monkeys. This cell line provides a marked improvement over the currently available testing strategies.
Claims11
A method of detecting African Swine Fever Virus (ASFV) in a sample, comprising the steps of:
- a. contacting the sample with a culture of Ma-104 cells under conditions that allow for infection of the cells by ASFV;
- b. incubating the Ma-104 cell and sample mixture under conditions that allow for ASFV detection;
- and
- c. analyzing the mixture from step b for the presence or absence of replicating ASFV.
A composition comprising Ma-104 cells, African Swine Fever Virus (ASFV), and red blood cells.
Dependencies are inferred from the claim text.