Microfluidic device and a method for provision of double emulsion droplets

A microfluidic device for production of double emulsion droplets comprising:

• a microfluidic section comprising a plurality of microfluidic units;
• and
• a well section comprising a plurality of groups of wells comprising one group of wells for each microfluidic unit;
• each microfluidic unit comprising a fluid conduit network comprising:
• a plurality of supply conduits comprising a primary supply conduit, a secondary supply conduit, and a tertiary supply conduit;
• a transfer conduit comprising a first transfer conduit part having a first affinity for water, wherein the first affinity for water is hydrophobic;
• a collection conduit comprising a first collection conduit part having a second affinity for water being different from the first affinity for water, wherein the second affinity for water is hydrophilic;
• a first fluid junction providing fluid communication between the primary supply conduit, the secondary supply conduit, and the transfer conduit, the first transfer conduit part extending from the first fluid junction;
• and
• a second fluid junction providing fluid communication between the tertiary supply conduit, the transfer conduit, and the collection conduit, the first collection conduit part extending from the second fluid junction;
• each group of wells comprising a plurality of wells comprising a collection well and a plurality of supply wells comprising a primary supply well and a tertiary supply well,
• the well section and the microfluidic section being fixedly connected to each other such that each group of wells being fixedly connected to a respective corresponding microfluidic unit, wherein for each group of wells:
• the collection well is in fluid communication with the collection conduit of the corresponding microfluidic unit;
• the primary supply well is in fluid communication with the primary supply conduit of the corresponding microfluidic unit;
• the tertiary supply well is in fluid communication with the tertiary supply conduit of the corresponding microfluidic unit;
• and
• one supply well of the plurality of supply wells is in fluid communication with the secondary supply conduit of the corresponding microfluidic unit,
• wherein the microfluidic device is configured for containing liquids in an amount required for the provision of droplets as well as the resulting droplets, and further wherein the microfluidic device is a single use device and produces double emulsion droplets.