Microfluidic device and a method for provision of emulsion droplets

A microfluidic device comprising:

• a microfluidic section comprising one or more microfluidic units;
• and
• a well section comprising one or more groups of wells comprising one group of wells for each microfluidic unit;
• the well section and the microfluidic section forming a fixedly connected unit such that each group of wells forms a fixedly connected unit with a respective corresponding microfluidic unit,
• each microfluidic unit comprising a fluid conduit network comprising:
• a plurality of supply conduits comprising a secondary supply conduit and a primary supply conduit comprising a capillary structure having a volume of at least 2 μL, wherein the secondary supply conduit comprises a first secondary supply conduit and a second secondary supply conduit, wherein the first secondary supply conduit and the second secondary supply conduit are configured to exert a pinching action of a second fluid on a stream of a first fluid from the primary supply conduit during use of the microfluidic device;
• a transfer conduit;
• and
• a first fluid junction providing fluid communication between the primary supply conduit, the first secondary supply conduit, the second secondary supply conduit, and the transfer conduit;
• each group of wells comprising a plurality of wells comprising a collection well and one or more supply wells comprising a primary supply well,
• the collection well being in fluid communication with the transfer conduit of the corresponding microfluidic unit,
• the primary supply well being in fluid communication with the primary supply conduit and the secondary supply conduit of the corresponding microfluidic unit,
• wherein the microfluidic device further comprises a base microfluidic piece and a base well structure piece,
• the base microfluidic piece forming a base part of the microfluidic section and being provided with a first planar surface having a plurality of ramified recesses providing a base part of each fluid conduit network of the microfluidic device,
• the base well structure piece forming a base part of the well section and forming a second planar surface facing the first planar surface of the base microfluidic piece,
• the second planar surface forming part of the microfluidic section and providing a capping part of each fluid conduit network of the microfluidic device.

A microfluidic device comprising:

• a microfluidic section comprising one or more microfluidic units;
• and
• a well section comprising one or more groups of wells comprising one group of wells for each microfluidic unit;
• the well section and the microfluidic section forming a fixedly connected unit such that each group of wells forms a fixedly connected unit with a respective corresponding microfluidic unit,
• each microfluidic unit comprising a fluid conduit network comprising:
• a plurality of supply conduits comprising a secondary supply conduit and a primary supply conduit comprising a capillary structure having a volume of at least 2 μL, wherein the secondary supply conduit comprises a first secondary supply conduit and a second secondary supply conduit, wherein the first secondary supply conduit and the second secondary supply conduit are configured to exert a pinching action of a second fluid on a stream of a first fluid from the primary supply conduit during use of the microfluidic device;
• a transfer conduit;
• and
• a first fluid junction providing fluid communication between the primary supply conduit, the first secondary supply conduit, the second secondary supply conduit, and the transfer conduit;
• each group of wells comprising a plurality of wells comprising a collection well and one or more supply wells comprising a primary supply well,
• the collection well being in fluid communication with the transfer conduit of the corresponding microfluidic unit,
• the primary supply well being in fluid communication with the primary supply conduit and the secondary supply conduit of the corresponding microfluidic unit,
• wherein the plurality of supply conduits of each fluid conduit network comprises a tertiary supply conduit,
• each microfluidic unit comprising a collection conduit and a second fluid junction, the second fluid junction of each microfluidic unit providing fluid communication within the corresponding fluid conduit network between the tertiary supply conduit, the transfer conduit, and the collection conduit,
• the transfer conduit of each fluid conduit network comprising a first transfer conduit part having a first affinity for water and extending from the corresponding first fluid junction, the collection conduit of each fluid conduit network comprising a first collection conduit part extending from the corresponding second fluid junction and having a second affinity for water being different from the first affinity for water,
• the one or more supply wells of each group of wells comprising a tertiary supply well being in fluid communication with the tertiary supply conduit of the corresponding microfluidic unit,
• the collection well being in fluid communication with the transfer conduit of the corresponding microfluidic unit via the collection conduit of the corresponding microfluidic unit.