Microfluidic analysis chip having negative pressure generation part and method for using same

A microfluidic analysis chip comprising:

• a main channel microtube for providing a space in which a sample injected from a sample inlet hole formed at one end reacts to a reagent while moving toward the other end;
• a chip housing enclosing the main channel microtube;
• a negative pressure generation unit located inside the chip housing and connected to affect internal pressure of the main channel microtube;
• a main exhaust hole formed on any one side of the main channel microtube to move internal air of the main channel microtube and external air of the chip housing to each other;
• and
• a main exhaust stopper for opening the main exhaust hole when the negative pressure generation unit moves in a direction increasing the internal pressure of the main channel microtube, and closing the main exhaust hole when the negative pressure generation unit moves in a direction decreasing the internal pressure of the main channel microtube.

A microfluidic analysis chip comprising:

• a main channel microtube for providing a space in which a sample injected from a sample inlet hole formed at one end reacts to a reagent while moving toward the other end;
• a chip housing enclosing the main channel microtube;
• at least one or more subchannel microtubes, one end of which is connected to a side surface of the main channel microtube, into which a reagent is injected;
• a negative pressure generation unit located inside the chip housing and connected to affect internal pressure of the main channel microtube;
• a sample inlet stopper for opening and closing the sample inlet hole;
• a sub-exhaust hole formed at one end of the subchannel microtube to move internal air of the subchannel microtube and external air of the chip housing to each other;
• and
• a sub-exhaust stopper for opening and closing the sub-exhaust hole.

A method of using a microfluidic analysis chip having a pushed unit and a pressed unit of an elastic material, the method comprising the steps of:

• (a) injecting a sample into a sample inlet hole;
• and
• (b) pressing a pressure adjustment unit toward the inside of a main channel microtube, and pushing the pushed unit toward the outside of the main channel microtube together, by applying a force to the pressed unit,
• wherein, when an external force is applied to the pressed unit, the pressed unit is configured to be pressed toward the inner space of the main channel microtube, the pushed unit is configured to be pushed together with the pressed unit toward the outside of the main channel microtube, shapes of the pressed unit and the pushed unit are transformed together, and an internal pressure of the main channel microtube is unchanged, and
• wherein, when a pressure to the pushed unit by the external force exceeds a critical point, a shape of the pushed unit is configured to be transformed and fixed toward the outside of the microtube to be symmetrical in a direction opposite to a pushing point by the external force.

A method of using a microfluidic analysis chip having a pressed unit and a pushed unit, the method comprising the steps of:

• (a) injecting a sample into a sample inlet hole;
• (b) pressing the pressed unit toward the inside of a main channel microtube, and pushing the pushed unit toward the outside of the main channel microtube together, by applying a force to the pressed unit;
• and
• (c) restoring a shape in a direction of decreasing internal pressure of the main channel microtube by contacting the pressed unit,
• wherein, when an external force is applied to the pressed unit, the pressed unit is configured to be pressed toward the inner space of the main channel microtube, the pushed unit is configured to be pushed together with the pressed unit toward the outside of the main channel microtube, shapes of the pressed unit and the pushed unit are transformed together, and an internal pressure of the main channel microtube is unchanged, and
• wherein, when a pressure to the pushed unit by the external force exceeds a critical point, a shape of the pushed unit is configured to be transformed and fixed toward the outside of the microtube to be symmetrical in a direction opposite to a pushing point by the external force.

A method of using a microfluidic analysis chip having a sample inlet stopper, a main exhaust stopper, a sub-exhaust stopper, and a negative pressure generation unit, the method comprising the steps of:

• (a) injecting a sample into a sample inlet hole;
• (b) selectively opening and closing the sample inlet stopper, the main exhaust stopper or the sub-exhaust stopper;
• and
• (c) moving a sample or a reagent by handling the negative pressure generation unit.