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US 11,579,149 B2Granted
Hippo pathway bioluminescent biosensor
AssigneeQueen's University at Kingston
InventorsXiaolong Yang, Taha Azad, Kazem Nouri
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AssigneeQueen's University at Kingston
InventorsXiaolong Yang, Taha Azad, Kazem Nouri
Estimating the term…
Bioluminescent biosensors useful for monitoring and/or quantifying, in vitro or in vivo, activity of the Hippo signaling pathway. The biosensors monitor LATS kinase activity or YAP-TEAD interaction. The biosensors may be used in methods for monitoring and/or quantifying in real-time, in vitro or in vivo, activity of the Hippo signaling pathway, wherein the activity may be LATS kinase activity and/or YAP-TEAD interaction. The biosensors may be provided in kits for monitoring and/or quantifying in real-time, in vitro or in vivo, activity of the Hippo signaling pathway, wherein the activity may be LATS kinase activity and/or YAP-TEAD interaction.
A DNA construct comprising a nucleic acid sequence encoding a fusion protein comprising a fragment of firefly luciferase fused to at least one fragment of YAP.
A DNA construct comprising a nucleic acid sequence encoding a fusion protein comprising a fragment of firefly luciferase fused to at least one fragment of 14-3-3 protein, wherein the at least one fragment of 14-3-3 protein is capable of binding to YAP.
A DNA construct comprising a nucleic acid sequence encoding a fusion protein comprising a fragment of NanoBiT luciferase fused to at least one fragment of YAP.
A DNA construct comprising a nucleic acid sequence encoding a fusion protein comprising a fragment of NanoBiT luciferase fused to at least one fragment of 14-3-3 protein, wherein the at least one fragment of 14-3-3 protein is capable of binding to YAP.
A DNA construct comprising a nucleic acid sequence encoding a fusion protein comprising a fragment of NanoBit luciferase that is LgBiT comprising amino acid sequence SEQ ID NO:52 and a fragment of YAP comprising amino acids 50-171 of SEQ ID NO:2.
A DNA construct comprising a nucleic acid sequence encoding a fusion protein comprising a fragment of NanoBit luciferase that is SmBiT comprising amino acid sequence SEQ ID NO:54 and a fragment of YAP comprising amino acids 50-171 of SEQ ID NO:2.
A DNA construct comprising a nucleic acid sequence encoding a fusion protein comprising a fragment of NanoBiT luciferase fused to at least one fragment of TEAD protein, wherein the at least one fragment of TEAD protein is capable of binding to YAP.
Dependencies are inferred from the claim text.