Methods for analysing a urine sample
AssigneeArquer Diagnostics Limited
InventorsDavid Nicholas Miller-Jones, Jacqueline Stockley, Cheryl Nyberg
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Abstract
The present invention provides methods for analysing a urine sample from a subject comprising exposing the urine sample to a lysis buffer which is capable of releasing at least one biomarker from cells in the urine sample. The present invention further provides kits, devices, and apparatuses that can be used in these methods. Finally, the present invention provides methods for detecting the presence of a urological cancer in a subject comprising performing an assay on a sample from a subject to determine the concentration of an Mcm protein.
Claims6
A method for analysing a urine sample from a subject comprising:
- a. exposing the urine sample to a lysis buffer wherein the lysis buffer is capable of releasing a minichromosome maintenance (Mcm) protein from cells in the urine sample, wherein the lysis buffer comprises (i) a detergent which comprises or consists of polyethylene glycol p-(1,1,3,3-tetramethylbutyl)-phenyl ether, and (ii) a buffer component which comprises or consists of tris(hydroxymethyl)methylamine or tris(hydroxymethyl)methylamine hydrochloride;
- and wherein the Mcm protein is Mcm5;
- and
- b. performing an enzyme-linked immunosorbent assay (ELISA) to determine the concentration of Mcm5 in the urine sample.
Dependencies are inferred from the claim text.