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US 11,478,541 B2Granted
Method for inactivating Zika virus and for determining the completeness of inactivation
AssigneeTakeda Vaccines, Inc.
InventorsJill A. Livengood, Holli Giebler, Hansi Dean, Tatsuki Satou, Raman Rao, Jackie Marks, Mark Lyons, Asae Shintani, James Gifford
Life of this patent
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Abstract
The present disclosure relates to methods for inactivating a Zika virus which can be used in vaccines and immunogenic compositions. The present disclosure also relates to a method for determining the completeness of inactivation of an arbovirus preparation.
Claims17
A method for determining the completeness of inactivation of an arbovirus preparation, comprising, the steps of:
- (i) inoculating, cultured insect cells with an arbovirus preparation which was subjected to an inactivation step and incubating the insect cells for a first period of time, thereby producing an insect cell supernatant;
- (ii) inoculating cultured mammalian cells with the insect cell supernatant produced in (i) and incubating the mammalian cells for a second period of time;
- and (iii) determining whether the arbovirus preparation contains a residual replicating virus that produces a cytopathic effect on the mammalian cells.
Dependencies are inferred from the claim text.