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US 11,442,073 B2Granted
Methods and systems for measuring serotonin in a sample
AssigneeLaboratory Corporation of America Holdings
InventorsMatthew Lee Francis Crawford, Yvonne Zamorano Wright, Russell Philip Grant
Life of this patent
Estimating the term…
Abstract
Disclosed are methods and systems for measuring serotonin in a sample using liquid chromatography and mass spectrometry.
Claims25
A method for determining amount of serotonin released by a biological sample, the method comprising:
- incubating at least one sample comprising the biological sample, heparin and donor platelets for a period of time to release serotonin from the donor platelets, thereby generating at least one incubated sample;
- chromatographically separating serotonin from other components in the at least one incubated sample using liquid chromatography;
- and
- analyzing the chromatographically separated serotonin by tandem mass spectrometry to determine the amount of released serotonin in the at least one sample relative to a total amount of serotonin available in the donor platelets.
1 more element
A system for determining amount of serotonin released by a biological sample, the system comprising:
- a station for incubating at least-one sample comprising the biological sample, heparin and donor platelets for a period of time to release serotonin from the donor platelets, thereby generating at least one incubated sample;
- a station for chromatographically separating serotonin from other components in the at least one incubated sample using liquid chromatography;
- and
- a station for analyzing the chromatographically separated serotonin by tandem mass spectrometry to determine amount of released serotonin in the at least one sample relative to a total amount of serotonin available within the donor platelets.
1 more element
Dependencies are inferred from the claim text.