US 11,442,066 B2 · Method for identifying drug-discovery target protein for development of antibody drug, and method for producing antibody against target protein

Method for identifying drug-discovery target protein for development of antibody drug, and method for producing antibody against target protein

AssigneesMitsui Chemicals, Inc., National Institutes of Biomedical Innovation, Health and Nutrition, Savid Therapeutics Inc.

InventorsTsuneji Suzuki, Yoshiyuki Totani, Kosuke Mano, Shinichi Banba, Haruhiko Kamada, Taisuke Nakayama, Hiroki Akiba, Kouhei Tsumoto, Tsuyoshi Inoue

Life of this patent

Estimating the term…

Abstract

Finding a protein of a minute amount present on a cell membrane to provide a method for producing an antibody against the protein. Producing an antibody using a protein identified by an identification method including: a labeling step of using a labeling agent comprising at least one selected from bis-iminobiotin compounds and bis-biotin compounds to obtain cells having a labeled protein; a degradation step of preparing a degradation product for an immobilization treatment, the degradation product containing the labeled protein; an immobilization step of immobilizing the labeled protein contained in the degradation product for an immobilization treatment on a stationary phase via a streptavidin mutant; a cleavage step of releasing an analysis sample from the stationary phase on which the labeled protein is immobilized; and an analysis step of analyzing the analysis sample to identify the labeled protein.

Claims3

Dependent claims

A bis-iminobiotin compound selected from the group consisting of compounds shown in following Tables A3 to A10:

TABLE A3
1
2
3
4
5
6
TABLE A4
1
2
3
4
5
6
7
TABLE A5
1
2
3
4
5
TABLE A6
1
2
3
4
5
TABLE A7
1
2
3
4
5
TABLE A8
1
2
3
4
5
TABLE A9
1
2
3
4
5
6
TABLE A10
1
2
3
4
5
6
wherein X is NH, J is sulfur, L is NH, G-Y is selected from the groups shown in following Table G2, wherein E means a bond to a partial structure in each of the compounds shown in the Tables A3 to A10, and Y is an N-hydroxysuccinimide ester or an N-hydroxysulfosuccinimide ester:
TABLE G2
1
2
3
4
8
9
11

59 more elements

Dependencies are inferred from the claim text.

Facts

Application

16/486,723Filed 19 Feb 2018

Priority

JP JP2017-02852017 Feb 2017

Published

US 2019/0383821 A119 Dec 2019

Granted

13 Sep 2022

Term ends

Not estimatedThe source records don’t support an estimate.

Classification16 codes

G01N 33/58
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing:…
C07D 207/40
Oxygen or sulfur atoms: 2,5-Pyrrolidine-diones
C07D 233/20
Having one double bond between ring members or between a ring member and a non-ring member: with substituted hydrocarbon radicals, directly…
C07D 487/04
In which the condensed system contains two hetero rings: ortho-condensed systems