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US 11,442,061 B2Granted
Reducing optical interference in a fluidic device
AssigneeLabrador Diagnostics LLC
InventorsIan Gibbons, Michael O'Connell
Life of this patent
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Abstract
This invention is in the field of medical devices. Specifically, the present invention provides portable medical devices that allow real-time detection of analytes from a biological fluid. The methods and devices are particularly useful for providing point-of-care testing for a variety of medical applications. In particular, the medical device reduces interference with an optical signal which is indicative of the presence of an analyte in a bodily sample.
Claims12
A system for detecting an analyte in a sample of bodily fluid, the system comprising:
- a sample collection unit configured to receive the sample of bodily fluid;
- an assay assembly in fluidic communication with the sample collection unit, the assay assembly comprising a reagent chamber, a reaction site, and a fluidic channel that connects the reagent chamber with the reaction site, wherein a reactant is immobilized in the reaction site, and wherein the reagent chamber comprises a reagent;
- a waste chamber in fluidic communication with, but spaced apart from, the assay assembly, wherein the waste chamber comprises absorbent material configured to absorb waste liquids from the assay assembly such that optical interference of a fluorescent signal from the waste liquids is reduced, wherein the absorbent material is impregnated with a quenching agent, wherein the quenching agent inhibits an enzymatic reaction between the reagent and a second reagent, thereby reducing the optical interference;
- and
- a reader assembly comprising a controller, a detection assembly, and an actuating element configured to effect movements between the sample collection unit, the assay assembly, and the waste chamber, wherein the movements include:
- moving the sample through the sample collection unit to the assay assembly, such that the sample is allowed to react with the reactant immobilized in the reaction site;
- moving unreacted reactant from the assay assembly to the waste chamber;
- and
- moving the reagent from the reagent chamber to the reaction site through the fluidic channel,
- wherein the assay assembly is configured to yield a fluorescent signal indicative of the presence of the analyte in the sample, wherein the fluorescent signal results from a reaction between the sample and at least one of the reactant or the reagent;
- and
- wherein the detection assembly is configured to detect the analyte in the sample by detecting the fluorescent signal.
9 more elements
A fluidic device having a housing and being configured to run an assay for detecting an analyte in a sample of bodily fluid within the housing, the fluidic device comprising:
- a sample collection unit configured to receive the sample of bodily fluid;
- an assay assembly in fluidic communication with the sample collection unit, the assay assembly comprising a reagent chamber, a reaction site, a first fluidic channel that connects the sample collection unit to the assay assembly, a second fluidic channel that connects the assay assembly to the waste chamber, and a third fluidic channel that connects the reagent chamber with the reaction site, wherein a reactant is immobilized in the reaction site, and wherein the reagent chamber comprises a reagent;
- and
- a waste chamber in fluidic communication with, but spaced apart from, the assay assembly, wherein the waste chamber comprises absorbent material configured to absorb waste liquids from the assay assembly such that optical interference of a fluorescent signal from the waste liquids is reduced, wherein the absorbent material is impregnated with a quenching agent, wherein the quenching agent inhibits an enzymatic reaction between the reagent and a second reagent, thereby reducing the optical interference,
- wherein the assay assembly is configured to:
- receive the sample from the sample collection unit, wherein the sample is moved from the sample collection unit to the assay assembly through the first fluidic channel;
- allow the sample to react with the reactant immobilized in the reaction site, wherein unreacted reactant is moved to the waste chamber through the second fluidic channel;
- allow the reagent from the reagent chamber to react at the reaction site, wherein the reagent is moved from the reagent chamber to the reaction site through the third fluidic channel;
- and
- yield a fluorescent signal indicative of the presence of the analyte in the sample, wherein the fluorescent signal results from a reaction between the sample and at least one of the reactant or the reagent.
7 more elements
Dependencies are inferred from the claim text.