Methods and systems for determining a pregnancy-related state of a subject

A method for treating a pregnant subject for a pregnancy complication, comprising:

• (a) assaying nucleic acid molecules obtained from a cell-free blood sample of said pregnant subject to determine at least one ribonucleic acid (RNA) level of at least one pregnancy-associated gene, wherein said at least one pregnancy-associated gene is differentially expressed in a first population of subjects with said pregnancy complication as compared to a second population of subjects without said pregnancy complication, wherein said assaying comprises selectively enriching said nucleic acid molecules for said at least one pregnancy-associated gene with nucleic acid primers or probes having sequence complementarity with a nucleic acid sequence of said at least one pregnancy-associated gene;
• (b) computer processing said at least one RNA level of said at least one pregnancy-associated gene determined in (a) (i) against at least one reference RNA level of said at least one pregnancy-associated gene or (ii) with a trained machine learning algorithm;
• (c) determining, based at least in part on said computer processing in (b), that said pregnant subject has or is at an elevated risk of having said pregnancy complication;
• and
• (d) providing a therapeutic intervention to said pregnant subject to treat said pregnancy complication, based at least in part on said determining in (c).