Method for assaying the activity of spermidine/spermine N1-acetyltransferase
AssigneeBioMark Technologies Inc.
InventorsBrian Cheng, Rashid Bux, Daniel Sitar
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Abstract
A method for assaying activity of the enzyme spermidine/spermine N1-acetyltransferase (SSAT) uses SSAT substrates by detecting their acetylated forms. SSAT substrates may include rimantadine and tocainide wherein their metabolism occurs in part by the action of the inducible enzyme SSAT to produce the acetylated metabolites N-acetylrimantadine and N-acetyltocainide respectively. SSAT activity may be correlated to pathologic conditions.
Claims10
A method for determining activity of spermine/spermidine N1-acetyltransferase (SSAT) in a mammal, the method comprising:
- (a) providing a biological sample from the mammal obtained 2-24 hours following administration of a single dose of rimantadine;
- and;
- (b) quantifying the level of N-acetyl rimantadine in the biological sample, thereby determining SSAT activity in the mammal.
A method for determining activity of spermine/spermidine N1-acetyltransferase (SSAT) in a mammal, the method comprising:
- (a) administering a single dose of rimantadine to the mammal;
- (b) collecting a biological sample from the mammal 2-24 hours following the single dose rimantadine administration;
- and
- (c) quantifying the level of N-acetyl rimantadine in the biological sample, thereby determining SSAT activity in the mammal.
Dependencies are inferred from the claim text.