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US 11,441,164 B2Granted
Biosynthetic production of psilocybin and related intermediates in recombinant organisms
AssigneeCB Therapeutics, Inc.
InventorsJacob Michael Vogan, Laura Flatauer Peiffer, James Lee Wade, Tyrone Jacob Yacoub, Kirsten Tang, Rachel Nadine Burnett
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Abstract
The systems and methods herein include engineering a host to produce psilocybin using engineered enzymes, genetic changes, and exogenous psilocybin precursor addition (e.g., addition of L-tryptophan to a growing culture of a psilocybin producing recombinant host strain). The process occurs in genetically engineered host cell(s) that can produce psilocybin.
Claims12
A recombinant host organism comprising:
- a plurality of cells transfected by a gene expressed in the recombinant host organism;
- wherein the recombinant host organism is a fungal species selected from the group consisting of Schizosaccharomyces cerevisiae, Schizosaccharomyces japonicus, Schizosaccharomyces pombe, Schizosaccharomyces cryophilus, Saccharomyces cerevisiae, Kluyveromyces lactis, Kluyveromyces dobzhanskii, and Yarrowia lipolytica;
- wherein the gene is codon optimized for expression in the recombinant host organism and is selected from a group consisting of PsiD, PsiH, PsiK, and PsiM;
- wherein:
- PsiD encodes an L-tryptophan decarboxylase and comprises nucleic acid sequence SEQ ID NO:1, SEQ ID NO:2, or SEQ ID NO:3;
- PsiH encodes a tryptamine 4-monooxygenase and comprises nucleic acid sequence SEQ ID NO:4, SEQ ID NO:5, or SEQ ID NO:6;
- PsiK encodes a 4-hydroxytryptamine kinase and comprises nucleic acid sequence SEQ ID NO:7 or SEQ ID NO:8;
- and
- PsiM encodes a methyl transferase and comprises codon optimized nucleic acid sequences SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, or SEQ ID NO:13.
6 more elements
Dependencies are inferred from the claim text.