Enzyme-catalyzed synthesis of (1S,5R)-bicyclolactone

A method of synthesizing (1S,5R)-bicyclolactone, comprising:

• (1) constructing a first genetically-engineered bacterium containing a Baeyer-Villiger monooxygenase gene and a second genetically-engineered bacterium containing a glucose dehydrogenase gene;
• (2) preparing a first suspension of resting cells of the first genetically-engineered bacterium and a second suspension of resting cells of the second genetically-engineered bacterium;
• (3) preparing a first supernatant containing Baeyer-Villiger monooxygenase and a second supernatant containing glucose dehydrogenase;
• and
• (4) mixing the first supernatant and the second supernatant to obtain a mixed supernatant;
• mixing the mixed supernatant with a raceme of a substituted bicyclo[3.2.0]-hept-2-en-6-one, a solvent, a hydrogen donor and a cofactor for an asymmetric Baeyer-Villiger oxidation to produce the (1S,5R)-bicyclolactone;
• wherein the Baeyer-Villiger monooxygenase has the amino acid sequence as shown in SEQ ID NO:1, or an amino acid sequence having an identity of more than 90% to SEQ ID NO:1.