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US 11,441,160 B2Granted
Compositions and methods for viral delivery of neoepitopes and uses thereof
AssigneeNant Holdings IP, LLC
InventorsPatrick Soon-Shiong, Shahrooz Rabizadeh, Kayvan Niazi
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Abstract
Cancer immunotherapy is enhanced by co-expression of cancer associated or tumor-specific (neo)epitopes with co-stimulatory molecules and/or other immune activators. Where desired, treatment may be enhanced by administration of a immune checkpoint inhibitor.
Claims13
A method of generating a recombinant nucleic acid, comprising:
- assaying deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and inferred protein from matched tumor and histologically normal tissue samples from a patient to identify a patient-specific cancer-related neoepitope of the patient;
- determining binding of the patient-specific cancer-related neoepitope to an HLA-type of the same patient, and determining an expression level of the patient-specific cancer-related neoepitope, wherein the determination of expression level uses mass spectroscopy based sequencing;
- selecting at least one co-stimulatory molecule;
- and
- genetically modifying an expression vector to include a nucleic acid encoding the at least one costimulatory molecule and the patient-specific cancer-related neoepitope, wherein the expression vector is an adenoviral or yeast or bacterial expression vector.
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Dependencies are inferred from the claim text.