Vitis vinifera with reduced MLO6 and MLO11 expression and increased resistance to powdery mildew
AssigneeFondazione Edmund Mach
InventorsMickael Malnoy, Stefano Pessina, Riccardo Velasco, Michele Perazzolli, Luisa Lenzi
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Abstract
Provided herein are Vitis vinifera exhibiting Erysiphe necator resistance. In particular, provided herein are Vitis vinifera having in their genome mildew resistance locus O (MLO) genes, in particular and MLO6 gene and an MLO11 gene, where the MLO6 gene and MLO11 gene have reduced expression and/or function.
Claims8
An isolated Vitis vinifera comprising in its genome:
- a first modification to a VvMLO6 gene encoding a protein having an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 3, the modification to the VvMLO6 gene comprising one or more non-natural mutations, insertions, substitutions, or deletions, wherein the modification results in a reduction in expression of the protein having the amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 3 compared to a Vitis vinifera lacking said modification in said VvMLO6 gene encoding the protein having an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 3;
- and
- a second modification to a VvMLO11 gene encoding a protein having an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 2, the modification to the VvMLO11 gene comprising one or more non-natural mutations, insertions, substitutions, or deletions, wherein the modification results in a reduction in expression of the protein having the amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 2 compared to a Vitis vinifera lacking said modification in said VvMLO11 gene encoding the protein having an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 2,
- wherein said Vitis vinifera having said modifications exhibits a reduction in powdery mildew disease severity compared to a Vitis vinifera lacking said modification modifications in said VvMLO6 gene and said VvMLO11 gene.
A method for obtaining a Vitis vinifera having resistance to powdery mildew comprising introducing a modification comprising one or more non-natural mutations, insertions, substitutions, or deletions to a VvMLO6 gene encoding a protein having at least 95% sequence identity to SEQ ID NO:
- 3 in a Vitis vinifera, wherein the modification results in:
- a decrease of function, loss of function, reduced expression, or absence of a protein having at least 95% sequence identity to SEQ ID NO:
- 3 compared to a Vitis vinifera lacking said modification in said VvMLO6 gene;
- and
- introducing a second modification comprising one or more non-natural mutations, insertions, substitutions, or deletions to a VvMLO11 gene encoding a protein having at least 95% sequence identity to SEQ ID NO:
- 2 in a Vitis vinifera, wherein the second modification results in:
- a decrease of function, loss of function, reduced expression, or absence of a protein having at least 95% sequence identity to SEQ ID NO:
- 2 compared to a Vitis vinifera lacking said modification in said VvMLO11 gene.
Dependencies are inferred from the claim text.