CRISPR/cas systems for c1-fixing bacteria
AssigneeLanzaTech NZ, Inc.
InventorsShilpa Nagaraju, Michael Koepke
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Abstract
The invention provides methods of genetically engineering a C1-fixing bacterium using a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) (CRISPR/Cas) system. Preferably, the Cas protein is under the control of an inducible promoter.
Claims16
A method of genetically engineering a C1-fixing bacterium comprising introducing into a C1-fixing bacterium containing a DNA molecule comprising a target sequence an engineered, non-naturally occurring Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) (CRISPR/Cas) system comprising one or more vectors comprising:
- (a) a nucleotide sequence encoding a guide RNA that hybridizes with the target sequence and
- (b) a nucleotide sequence encoding a type-II Cas9 protein under the control of an inducible promoter;
- wherein the inducible promoter is ipl12;
- wherein the Cas9 interacts with the DNA molecule;
- and
- wherein expression of a gene product or a nucleotide sequence of the DNA molecule is altered.
Dependencies are inferred from the claim text.