Methods for the manufacture of proteolytically processed polypeptides
AssigneeIpsen Bioinnovation Limited
InventorAndreas Rummel
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Abstract
A proteolytically active polypeptide comprising an acid sequence having at least 50% sequence identity with the sequence of SEQ ID NO: 1 with the proviso that the polypeptide does not comprise the amino acid sequence of SEQ ID NO: 1. A nucleic acid encoding the polypeptide. An antibody specifically binding to the polypeptide. A method for the manufacture of a proteolytically processed polypeptide comprising contacting a first polypeptide having at least 50% sequence identity to SEQ ID NO: 1 with a second polypeptide that is susceptible to proteolysis by said first polypeptide.
Claims20
A method for producing a proteolytically processed polypeptide, the method comprising contacting a first polypeptide having at least 50% sequence identity with SEQ ID NO:
- 1 with a second polypeptide that is susceptible to proteolysis by the first polypeptide, resulting in the proteolytic processing of the second polypeptide into at least two cleavage products;
- wherein:
- the method occurs in vitro;
- and/or at least one of the first or second polypeptide is recombinant or purified.
A method for proteolytically processing a polypeptide in a subject wherein a first polypeptide having at least 50% sequence identity with SEQ NO:
- 1 and a second polypeptide that is susceptible to proteolysis by the first polypeptide are both administered to a subject and allowed to contact each other, resulting in the proteolytic processing of the second polypeptide into at least two cleavage products.
Dependencies are inferred from the claim text.