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US 11,441,132 B2Granted
Mutated sleeping beauty transposase
AssigneeEuropean Molecular Biology Laboratory
InventorsOrsolya Barabas, Irma Querques, Cecilia Ines Zuliani
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AssigneeEuropean Molecular Biology Laboratory
InventorsOrsolya Barabas, Irma Querques, Cecilia Ines Zuliani
Estimating the term…
The present invention relates to improved transposase polypeptides having increased solubility. The enzyme of the invention was developed based on the Sleeping Beauty (SB) transposase. The invention provides further nucleic acids, vectors and recombinant cells encoding or containing the improved transposase, as well as a transposase system. Furthermore provided are medical and non-medical uses of the transposase of the invention for gene delivery. The invention is in particular useful as a tool for gene delivery in genetically modified cell based therapeutic approaches for treating various diseases.
A transposase polypeptide, wherein the transposase polypeptide is a Sleeping Beauty (SB) transposase and comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO:2 but with at least one mutated amino acid residue at position 176 or position 212 of SEQ ID NO:2.
Dependencies are inferred from the claim text.