Heparosan synthases and use thereof for saccharide synthesis
AssigneeThe Regents of the University of California
InventorsXi Chen, Lan Na, Hai Yu, John B. McArthur
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Abstract
Heparosan synthase variants having improved expression levels, enhanced thermal stability, and/or reduced reverse glycosylation activity are provided. Methods for making oligosaccharides and polysaccharides, including heparin analogs and heparan sulfate analogs, are also described.
Claims17
A Pasteurella multocida heparosan synthase 2 (PmHS2) variant consisting essentially of a polypeptide having at least 90% identity to the amino acid sequence set forth in SEQ ID NO:1, which comprises a mutation at one or more positions corresponding to Y165, R184, L185, F187, I188, T189, D211, N237, K379, L462, T464, T466, V467, S485, G486, M487, and D489 in SEQ ID NO:1.
A Pasteurella multocida heparosan synthase 2 (PmHS2) variant consisting essentially of a polypeptide having at least 90% identity to the amino acid sequence set forth in SEQ ID NO:3, which comprises a mutation at one or more positions corresponding to Y176, R195, L196, F198, I199, T200, D222, N248, K390, L473, T475, T477, V478, S496, G497, M498, and D500 in SEQ ID NO:3.
Dependencies are inferred from the claim text.