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US 11,441,121 B2Granted
Spheroid cell culture article and methods thereof
AssigneeCorning Incorporated
InventorsScott Matthew Bennett, Brian Robb Douglass, Paul Ernest Gagnon, Jr., Gregory Roger Martin, Paul Michael Szlosek, Allison Jean Tanner
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Abstract
A spheroid cell culture article including: a frame having a chamber including: an opaque side wall surface; a top aperture; a gas-permeable, transparent bottom; and optionally a chamber annex surface and second bottom, and at least a portion of the transparent bottom includes at least one concave arcuate surface, is disclosed. Methods of making and using the article are also disclosed.
Claims20
A cell culture article comprising:
- a chamber comprising:
- an angled side wall;
- a top aperture;
- and
- a gas-permeable, liquid impermeable bottom comprising at least one concave surface, wherein at least a portion of the bottom is transparent and wherein at least a portion of the bottom comprises a low-adhesion or no-adhesion material in or on the at least one concave surface, wherein the angled side wall has a decreasing diameter from the chamber's top aperture to the bottom;
- and
- a chamber annex for receiving a pipette tip for aspiration, the chamber annex comprising an integral surface adjacent to and in fluid communication with the chamber, wherein the chamber annex is not physically separated from the chamber, the chamber annex having a second bottom spaced away and at an elevation above the gas-permeable, liquid impermeable bottom, wherein the second bottom deflects fluid dispensed from a pipette away from the gas-permeable, liquid impermeable bottom, and wherein the chamber annex integral surface is angled with the chamber's angled side wall.
4 more elements
Dependencies are inferred from the claim text.