Culture media comprising n-Acyl-X-glutamine dipeptides

A cell culture media, comprising

• L-glutamine from a set of N-acylated dipeptides Acyl-XQ;
• and
• L-glutamine from a set of other glutamine-sources Qsource in a defined molar ratio R=n(Acyl-X-Q)/n(Qsource),
• wherein:
• X is defined as an L-amino acid;
• Q is defined as L-glutamine attached via an amide bond to L-amino acid X;
• Acyl is defined as a C1-C7-acyl moiety attached via an amide bond to the amino-terminus of L-amino acid X;
• R is defined to be in the range of 0.03 to 20;
• n(Acyl-X-Q) is the total amount of substance of L-glutamine contained in the set of N-acylated dipeptides Acyl-X-Q in the culture media;
• n(Qsource) is the total amount of substance of L-glutamine contained in the set of other glutamine sources Qsource in the culture media;
• the constituents of the set of other L-glutamine sources Qsource are selected from the following:
• Free L-glutamine, dipeptides Y-Q, or mixtures thereof, wherein Y is defined as one of the 20 genetically encoded L-amino acids, wherein Q is defined as L-glutamine attached via an amide bond to L-amino acid Y, and the amide bond connecting Y and Q in dipeptide Y-Q is a regular backbone amide bond involving the carboxy terminus of amino acid Y and the amino terminus of glutamine Q;
• and
• the cell culture media are serum free.