Oxime cross-linked biocompatible polymer hydrogels and methods of use thereof

A method of administering an oxime cross-linked bioadhesive hydrogel to a tissue for use as an in-situ anti-adhesion barrier comprising:

• administering to a living tissue of an individual an effective amount of a combination of a first polymer comprising an aminooxy group selected from a hydroxyl amine and an alkoxy amine, a second polymer comprising a reactive oxo group, and a third polymer comprising a catechol group, wherein the weight ratio of first polymer:second polymer:third polymer is 1:1:0.75-1,
• wherein the first polymer and second polymer are initially mixed and react to form an oxime cross-linked biocompatible hydrogel proximate to the tissue,
• wherein the hydrogel has a surface comprising a surface oxo group, and
• wherein the surface oxo group reversibly binds a surface amine on the tissue to form an imine.

A kit for making an oxime-linked bioadhesive hydrogel for use as an in-situ anti-adhesion tissue barrier comprising;

• a. a first polymer comprising an aminooxy group;
• b. a second polymer comprising a reactive oxo group;
• c. a third polymer comprising a catechol group;
• and
• d. instructions for combining the first, second, and third polymers to form the bioadhesive hydrogel, wherein the weight ratio of first polymer:second polymer:third polymer is 1:1:0.75-1.