Universal antibody-mediated biosensor
AssigneeUniversity of Maryland, Baltimore
InventorsDan Schulze, Scott E. Strome
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Abstract
A universal antibody-mediated biosensor is provided that comprise a biosensor cell line stably expressing a novel chimeric fusion protein that can be used to detect target agents in a sample. The fusion protein has an extracellular antibody-binding domain that binds antibodies without regard to their binding specificity and a signaling domain that induces cellular activation upon antigen binding. Because the fusion protein binds to the Fc region of any antibody, it can serve as a universal pathway between extracellular signaling and intracellular activation. The biosensor can be used to detect the presence of selected antigens in a sample.
Claims14
A biosensor cell stably expressing a chimeric fusion protein, wherein the chimeric fusion protein comprises an Fcγ receptor (FcγR) antibody-binding domain and a signaling domain, wherein the FcγR antibody-binding domain is an FcγRI antibody-binding domain, wherein the signaling domain is an immunoglobulin alpha (Igα) signaling domain, and wherein the fusion protein is the FcγRI/Igα fusion protein set forth in SEQ ID NO:8.
A chimeric fusion protein comprising an Fcγ receptor (FcγR) antibody-binding domain and a signaling domain, wherein the FcγR antibody-binding domain is an FcγRI antibody-binding domain, wherein the signaling domain is an Igα signaling domain, and wherein the fusion protein is the FcγRI/Igα fusion protein set forth in SEQ ID NO:8.
Dependencies are inferred from the claim text.