Cancer-treating recombinant protein that improves the activity of cytotoxic killer cells targeting cancer cells and use thereof
AssigneeGwangju Institute of Science and Technology
InventorsChang-Duk Jun, Bu Nam Jeon, Hey-Ran Kim
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Abstract
A cancer killer cell in which a therapeutic recombinant protein or recombinant protein which improves cytotoxic activity of the cancer killer cell is loaded. In addition, a pharmaceutical composition including the recombinant protein or a recombinant protein-loaded cancer killer cell is disclosed. Further, disclosed is a method for preparing a recombinant protein-loaded cancer killer cell.
Claims13
A recombinant protein for treating cancer consisting of an amino acid sequence of SEQ ID NO:
- 1, which is loaded in a cancer killer cell to improve cytotoxic activity of the cancer killer cell, the cancer killer cell being a tumor-infiltrated T lymphocyte, T cell, or a natural killer cell including a chimeric antigen receptor, wherein the recombinant protein is further fused with a protein transduction domain (PTD) consisting of an amino acid sequence of SEQ ID NO:
- 2.
A pharmaceutical composition for treating cancer, comprising:
- i) a recombinant protein for treating cancer consisting of an amino acid sequence of SEQ ID NO:
- 1, which is loaded in a cancer killer cell to improve cytotoxic activity of the cancer killer cell;
- wherein the recombinant protein is further fused with a protein transduction domain (PTD) consisting of an amino acid sequence of SEQ ID NO:
- 2, wherein the cancer killer cell comprises the recombinant protein, the cancer killer cell being a tumor-infiltrated T lymphocyte, T cell, or a natural killer cell including a chimeric antigen receptor.
A method for producing a recombinant protein-loaded cancer killer cell, comprising:
- (a) fusing a PTD consisting of an amino acid sequence of SEQ ID NO:
- 2 into an amino acid of SEQ ID NO:
- 1;
- and
- (b) introducing the fusion protein of (a) into a cancer killer cell, the cancer killer cell being a tumor-infiltrated T lymphocyte, T cell, or a natural killer cell including a chimeric antigen receptor.
A method for producing a recombinant protein-loaded cancer killer cell, comprising:
- transducing into a cancer killer cell using retrovirus comprising a polynucleotide encoding an amino acid sequence of SEQ ID NO:
- 1, fused with a protein transduction domain (PTD) consisting of an amino acid sequence of SEQ ID NO:
- 2, the cancer killer cell being a tumor-infiltrated T lymphocyte, T cell, or a natural killer cell including a chimeric antigen receptor.
Dependencies are inferred from the claim text.