US 11,439,926 B2 · Chromatography medium

Abstract

The present invention is within the field of chromatography. More precisely, it relates to a novel chromatography medium, namely a hydrophobic medium provided with different lids excluding molecules over a certain size due to the porosity of the hydrophobic medium and/or the porosity of the lid. The invention also relates to use of the separation medium for purification of large molecules, which do not enter the separation medium, as well as small molecules, which enter the separation medium and are eluted from there.

Claims6

Dependent claims

An agarose-based separation medium, comprising,

a hydrophobic porous core entity with octylamine ligands having a ligand density of greater than 90 μmole/mL;
and
a porous hydrophilic lid covering the whole outer part of the core entity,
wherein the lid only allows molecules under 60,000D to penetrate and absorb onto the hydrophobic octyl ligands of said core entity of the separation medium,
wherein the hydrophobic core entity allows adsorption of molecules under 60,000D at low ionic strength 0-1M in the pH interval 2-11.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

16/701,942Filed 3 Dec 2019 · division

Priority

SE 0800923-522 Apr 2008

Published

US 2020/0101398 A12 Apr 2020

Granted

13 Sep 2022

Term ends

Not estimatedThe source records don’t support an estimate.

Classification17 codes

B01D 15/327
Reversed phase: with hydrophobic interaction
B01J 20/28009
Characterised by their physical properties: magnetic properties
B01J 20/285
Porous sorbents: based on polymers
B01J 20/287
Phases chemically bonded to a substrate, e.g. to silica or to polymers: non-polar phases; Reversed phases

Family

PCT/SE2009/050406Parent of this division · filed 21 Apr 2009

Application15/183,526Parent of this division · filed 15 Jun 2016

US 10,493,380 B2Parent of this division · filed 3 Dec 2019

US 2020/0101398 A1Pre-grant publication · published 2 Apr 2020

Application12/988,707Parent of this division

US 2011/0045574 A1Published application

US 2017/0050121 A1Published application

US 11,439,926 B2This patent

From the US filing history. Foreign family members aren’t listed.

Chromatography medium

Life of this patent

Chromatography medium

Life of this patent

Abstract

Claims6