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US 11,439,166 B2Granted
Ground meat replicas
AssigneeImpossible Foods Inc.
InventorsRanjani Varadan, Sergey Solomatin, Celeste Holz-Schietinger, Elysia Cohn, Ariel Klapholz-Brown, Jennifer Woan-Yi Shiu, Aniket Kale, Jessica Karr, Rachel Fraser
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Abstract
This document relates to ground meat replicas, and more particularly to plant-based products that mimic ground meat, including the fibrousness, heterogeneity in texture, beefy flavor, and red-to-brown color transition during cooking of ground meat.
Claims14
A recombinant Pichia pastoris organism comprising:
- eight stably integrated genes encoding recombinant heme biosynthesis enzymes;
- and
- a stably integrated nucleic acid encoding a recombinant heme-containing protein selected from leghemoglobin and myoglobin,
- wherein the heme biosynthesis enzymes are Pichia pastoris ALA synthase, Pichia pastoris ALA dehydratase, Pichia pastoris porphobilinogen deaminase, Pichia pastoris uroporphyrinogen III synthase, Pichia pastoris uroporphyrinogen III decarboxylase, Pichia pastoris coproporphyrinogen oxidase, Pichia pastoris protoporphyrinogen III oxidase, and Pichia pastoris ferrochelatase.
1 more element
A method for preparing a food additive comprising recombinant heme-containing protein, the method comprising:
- providing a recombinant Pichia pastoris organism comprising:
- eight stably integrated genes encoding recombinant heme biosynthesis enzymes;
- and a stably integrated gene encoding a recombinant heme-containing protein selected from leghemoglobin and myoglobin under the control of a methanol inducible promoter, wherein the recombinant heme biosynthesis enzymes comprise Pichia pastoris ALA synthase, Pichia pastoris ALA dehydratase, Pichia pastoris porphobilinogen deaminase, Pichia pastoris uroporphyrinogen III synthase, Pichia pastoris uroporphyrinogen III decarboxylase, Pichia pastoris coproporphyrinogen oxidase, Pichia pastoris protoporphyrinogen III oxidase, and Pichia pastoris ferrochelatase;
- fermenting the recombinant Pichia pastoris organism in a fermentation broth;
- inducing expression of the heme-containing protein with methanol;
- purifying the recombinant heme-containing protein;
- and
- combining the purified recombinant heme-containing protein with a buffered solution.
5 more elements
Dependencies are inferred from the claim text.