Humanized transgenic animal
AssigneeBiocytogen Pharmaceuticals (Beijing) Co., Ltd.
InventorsYuelei Shen, Chaoshe Guo, Yanan Guo, Yang Bai, Rui Huang, Lei Zhao, Xiaofei Zhou, Meiling Zhang, Jiawei Yao
Life of this patent
Estimating the term…
Abstract
Provided are a humanized transgenic non-human animal, especially a rodent, in particular a transgenic mouse containing a human interleukin 17A (IL-17A) gene, a human gene 17RA (IL-17RA) and/or a human TNF-alpha gene, and a preparation method therefor and the use thereof.
Claims14
A genetically-modified, non-human animal whose genome comprises a sequence encoding a humanized IL-17RA protein at an endogenous IL-17RA gene locus, wherein the sequence encoding the humanized IL-17RA protein is operably linked to an endogenous IL-17RA regulatory element at an endogenous IL-17RA gene locus, wherein the animal comprises a replacement of a part of exon 2, exons 3-10 and a part of exon 11 of the endogenous IL-17RA gene, with a part of exon 2, exons 3-10 and a part of exon 11 of a human IL-17RA gene, wherein the non-human animal detectably expresses the humanized IL-17RA protein on the surface of spleen cells, and the humanized IL-17RA protein comprises an amino acid sequence that is at least 95% identical SEQ ID NO:
- 39.
A method for making a genetically-modified non-human animal, comprising:
- (a) replacing in a fertilized egg or an embryonic stem cell, at an endogenous IL-17RA gene locus, a part of exon 2, exons 3-10, and a part of exon 11 of endogenous IL-17RA gene with a part of exon 2, exons 3-10 and a part of exon 11 of a human IL-17RA gene;
- (b) transplanting the fertilized egg of step (a) into a female non-human animal or transplanting the embryonic stem cell of step (a) into a blastocyst, which is then transferred into a female non-human animal;
- and
- (c) obtaining a genetically-modified, non-human animal, wherein the non-human animal has genome that comprises a sequence encoding a humanized IL-17RA protein that is operably linked to an endogenous IL-17RA regulatory element at an endogenous IL-17RA gene locus.
Dependencies are inferred from the claim text.