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US 11,433,127 B2Granted
Non-toxigenic Clostridium difficile spores for use in oral vaccination
AssigneeUniversity of South Florida
InventorsXingmin Sun, Abraham Sonenshein
Life of this patent
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Abstract
Described are non-toxigenic Clostridium difficile strains and spores. Also described are vaccines comprising the Clostridium difficile spores. Further described are methods of preventing or treating a Clostridium difficile infection in a subject in need thereof.
Claims9
A method of treating or preventing Clostridium difficile bacterial infection in a subject in need thereof, comprising administering to the subject a vaccine that comprises a non-toxigenic Clostridium difficile strain comprising:
- a) an immunogenic protein comprising
- i) a glucosyltransferase domain of Clostridium difficile toxin TcdB;
- ii) a cysteine proteinase domain of Clostridium difficile toxin TcdB;
- iii) a receptor binding domain of Clostridium difficile toxin TcdA;
- and
- iv) a receptor binding domain of Clostridium difficile toxin TcdB,
- wherein the glucosyltransferase domain of Clostridium difficile toxin TcdB comprises a W102A amino acid substitution and a D288N amino acid substitution when compared to SEQ ID NO.:
- 5, and wherein the cysteine proteinase domain of Clostridium difficile toxin TcdB comprises a C698A amino acid substitution when compared to SEQ ID NO.:
- 5.
6 more elements
Dependencies are inferred from the claim text.