Vaccinia viral vectors encoding chimeric virus like particles

A recombinant modified vaccinia Ankara (MVA) viral vector comprising:

• (i) a first heterologous nucleic acid sequence encoding a chimeric protein comprising (a) a mucin-1 (MUC-1) antigenic peptide and (b) a transmembrane domain of a viral glycoprotein (GP) of Marburg virus, wherein the first heterologous nucleic acid sequence encodes an amino acid sequence comprising the amino acid sequence of SEQ ID NO:
• 15, or an amino acid sequence 98% identical thereto, and
• (ii) a second heterologous nucleic acid sequence encoding a viral Marburg virus VP40 matrix protein;
• wherein the first heterologous nucleic acid sequence and second heterologous nucleic acid sequence are under the control of one or more promoters compatible with poxvirus expression systems, and wherein upon expression, the chimeric protein and VP40 matrix protein are capable of assembling together to form virus like particles (VLPs).